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KMID : 1134820120410040456
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Journal of the Korean Society of Food Science and Nutrition
2012 Volume.41 No. 4 p.456 ~ p.461
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Anti-Inflammatory Activity of Austroinulin from Stevia rebaudiana in LPS-induced RAW264.7 Cells
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Byun Myung-Woo
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Abstract
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The leaves of Stevia rebaudiana are well-known in Japan, Korea, and China as a natural sweetener. Medicinal uses of this plant originated in Paraguay and Brazil in the form of aqueous decoctions of the leaves used as a contraceptive agent and for the treatment of hyperglycemia. In the present study, the antioxidant, anti-hypertension, and anti-inflammatory activities of S. rebaudiana extracts are investigated for their use in food. The biologically-active compound was isolated and purified from S. rebaudiana. The isolated compound was identified as austroinulin (C20H34O3; molecular weight 322) by mass, IR spectrophotometry, 1D, and 2D-NMR. Austroinulin was characterized as a diterpenoid possessing a 3-methylpenta-2,4-dienyl at C-9. When subjected to an inflammatory mediator inhibitory assay from lipopolysaccharide (LPS)-activated macrophages, the austroinulin inhibited the enhanced production of nitric oxide (NO) and inducible nitric oxide synthase (iNOS) expression (10 μg/mL＝67.9 and 45.1%, respectively). This was significant and dose-dependent. The results suggest that austroinulin from S. rebaudiana inhibited the NO and iNOS in RAW 264.7 cells.
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KEYWORD
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Stevia rebaudiana, austroinulin, RAW264.7, anti-inflammation
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